dna methylation pyrosequencing  (Pyrosequencing Inc)

Journal: PLoS ONE

Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

doi: 10.1371/journal.pone.0056931

Figure Lengend Snippet: (A) Diagram of the experimental design. The differences of the global methylation levels between donors, pre-HCT recipients and post-HCT recipients were assessed by a pyrosequencing based methylation assay of repetitive DNA elements (LINE1 and NBL2) in whole blood. (B) NBL2 ΔMet values between donors, pre-HCT recipients, and 1 month post-HCT recipients. (C) NBL2 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marks the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.8450). During the follow up of the transplant, the mean values barely deviated from the initial post-HCT ΔMet. (D) LINE1 ΔMet values between donors, pre-HCT recipients and 1 month post-HCT recipients. (E) LINE1 ΔMet mean values between donors and recipients up to 12 months post-transplant. The dotted line marked the ΔMet mean value between donors and 1 month post-HCT recipients (ΔMet = 5.383).

Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

Techniques: Methylation

Journal: PLoS ONE

Article Title: DNA Methylation Dynamics in Blood after Hematopoietic Cell Transplant

doi: 10.1371/journal.pone.0056931

Figure Lengend Snippet: Scatter plots showing DNA methylation in donors versus post-transplant recipients 1 month and 6 months post-HCT. Red dots represent CpG sites with methylation values altered more than 20% relative to donor values.

Article Snippet: Pyrosequencing-based DNA methylation analysis is relatively inexpensive, is very sensitive, and provides an interesting alternative to protein and mRNA detection in blood samples, allowing the development of novel biomarkers in a large array of human diseases.

Techniques: DNA Methylation Assay, Methylation

Journal: Frontiers in Veterinary Science

Article Title: Metallopeptidades 2 and 9 genes epigenetically modulate equine endometrial fibrosis

doi: 10.3389/fvets.2022.970003

Figure Lengend Snippet: Relative COL1A1, COL1A2 and COL3A1 mRNA transcripts in equine endometrium graded as Kenney and Doig's categories I, IIA, IIB and III. Bars represent mean ± SEM.

Article Snippet: Transcription of COL1A1, COL1A2, COL3A1, MMP2, MMP9, TIMP1 , and TIMP2 genes and DNA methylation pattern by pyrosequencing of COL1A1, MMP2, MMP9, TIMP1 genes were evaluated.

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Metallopeptidades 2 and 9 genes epigenetically modulate equine endometrial fibrosis

doi: 10.3389/fvets.2022.970003

Figure Lengend Snippet: Methylation of DNA (%) of (A) COL1A1 , (B) MMP2 and (C) MMP9 in equine endometrium graded as Kenney and Doig's categories I, IIA, IIB and III. Bars represent mean±SEM. The asterisks indicate significant differences between endometrial categories (* P < 0.05, ** P < 0.01).

Article Snippet: Transcription of COL1A1, COL1A2, COL3A1, MMP2, MMP9, TIMP1 , and TIMP2 genes and DNA methylation pattern by pyrosequencing of COL1A1, MMP2, MMP9, TIMP1 genes were evaluated.

Techniques: Methylation

Journal: Frontiers in Veterinary Science

Article Title: Metallopeptidades 2 and 9 genes epigenetically modulate equine endometrial fibrosis

doi: 10.3389/fvets.2022.970003

Figure Lengend Snippet: Methylation (%) of COL1A1 by CpG position in equine endometrial categories graded as Kenney and Doig's classification. Red arrows and asterisks indicate significant differences between CpG positions (* P < 0.05, ** P < 0.01, *** P < 0.001).

Article Snippet: Transcription of COL1A1, COL1A2, COL3A1, MMP2, MMP9, TIMP1 , and TIMP2 genes and DNA methylation pattern by pyrosequencing of COL1A1, MMP2, MMP9, TIMP1 genes were evaluated.

Techniques: Methylation